Device

Part:BBa_K561000:Experience

Designed by: HUA Yijie, PI Ruoxi, XIA Yu   Group: iGEM11_ZJU-China   (2011-09-29)

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Applications of BBa_K561000

The expression of YFP under aerobic conditions: The expression of YFP under aerobic conditions is increasing during the exponential phase. With the micro-aerobic promoter, its expression intensity is very low. The highest expression intensity is 1.15. The PoPS of our device is 0.4506.

The expression of YFP under aerobic conditions:

The expression of YFP under anaerobic conditions: Pvgb is a micro-aerobic promoter and it contributes to high expression of YFP under micro-aerobic conditions. It’s expression level at anaerobic condition is relatively low. The PoPS of this device under anaerobic condition is -0.375.

The expression of YFP under anaerobic conditions:

The expression of YFP under micro-aerobic conditions (2.5% oxygen): The expression of YFP with 2.5% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.28. The PoPS of our device at 2.5% oxygen level is 13.9023.

The expression of YFP under micro-aerobic conditions (2.5% oxygen):

The expression of YFP under micro-aerobic conditions (10%) : The expression of YFP with 10% oxygen concentration can reach to a relatedly high level. With the micro-aerobic promoter (Pvgb), its expression intensity at the exponential phase is 1.24. The PoPS of our device at 10% oxygen level is 2.0653.

The expression of YFP under micro-aerobic conditions (10%) :

User Reviews

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